Jan 21 1. Drosophila as a model organism in genetics (p. 1-7)*
             Patterns of inheritance- autosomal and sex-linked (p. 84-86) traits.

Experiment I: Inheritance of unknown traits in Drosophila (handout).

Jan 28 2. Independent assortment in maize F₂ and test cross ears (p.8-10 and handout).
             Isolation of DNA from bovine spleen (p. 155-160 and handout).

Feb 4 3. Chi-square goodness of fit hypothesis testing (p.27-28 and handout); application to 
           maize data. 
           Expt. I continued: Classify F₁ and set up F₁ X F₁ crosses).

Feb 11 4. Cellulose acetate electrophoresis of allozymes in a Drosophila population  
               (handout), application of Hardy Weinberg equilibrium principle to data (p.237-240).

Feb 18 5. Expt.I completed (Sex and classify F₂ flies). Discussion of results. 
               Experiment VI: Induction and isolation of lethal mutations in Drosophila (handout)

Feb 25 6. Experiment V: Linkage analysis in Drosophila- the two point testcross (handout) 
                Mitosis (p. 41- 49) and Human metaphase chromosomes/karyotypes (p. 95-119)

March 4 7. Expt VI continued (Set up F₁ X F₁ cross)
                   Chromosome behavior in meiosis in animals- oogenesis in Ascaris and spermatogenesis in the grasshopper
                   (p.51-58 and handout) and rat testes (handout)

March 11 8. Restriction endonuclease digestion and electrophoresis of Lambda bacteriophage DNA (p.161-171 and handout).
                   Expt. V continued (Set up reciprocal test crosses).

March 18 9. Spring Break

March 25 10. Expt. VI completed (Classify F₂ generation). Discussion of results.
                    Meiosis in plants- micro sporogenesis in lily (59-69).

April 1 11. PCR Experiment- isolation of student cheek cell DNA (p.177-184).
                 Expt V completed (Classify offspring produced by reciprocal test crosses and discuss results.)

April 8 12. PCR expt. continued (Set up amplification reactions in PCR cycler).
                 Tetrad analysis in Sordaria (p. 141-146).

April 15 13. PCR expt continued (Perform agarose gel electrophoresis of PCR-amplified DNA and determine student DNA
                     finger prints). 
                  Collect stature data for Expt. II

April 22 14. Experiment II: Analysis of human stature, a quantitative genetic trait (handout).

April 29 15. Lab final exam

Lab grade is based on the following:

25%: Six unannounced quizzes covering previously untested material and advanced reading assignments. The lowest quiz grade will be dropped.

15%: Problem sets and analyses of experiments

35%: Formal lab reports over to-be-determined experiments completed in lab

25%: Lab final exam